The Girls Night Out is returning to Bury St Edmunds in March, after it was postponed following the death of The Queen in September.
St Nicholas Hospice is calling for people to "pull on their best pyjamas and put in the miles" for their charity walk fundraiser.
Charlie De-Moore, the hospice's events manager, said: "Although the walk may be taking place at a different time of year, participants can still expect all of the features they have come to know and love.
"Seeing Angel Hill lit up with flashing bunny ears is always an amazing sight, and for myself and everyone at St Nic's, it is an incredible feeling to know that all of those people are there to raise funds and support our hospice."
On Saturday, March 4, walkers can take part in a Zumba warm-up on Angel Hill and set off on the 11.2 or six-mile route at around 6.30pm.
Ms De-Moore added: "If you haven't already, please sign up, and join this special walk.
"Every step and mile makes a difference to your community's hospice care."
The charity is not organising another Girls Night Out event for its usual date in September, but says plans are underway to mark its upcoming 40th anniversary next year.
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